fluorescence elisa reader  (BMG Labtech)

Journal: Journal of Pharmaceutical Analysis

Article Title: Impact of FASN-enriched EVs on endothelial cell function in obstructive sleep apnea hypopnea syndrome

doi: 10.1016/j.jpha.2025.101251

Figure Lengend Snippet: The impact of serum-derived extracellular vesicles (s-EVs) on lung endothelial function in patients with obstructive sleep apnea hypopnea syndrome (OSAHS). (A) Distribution of s-EVs labeled with PKH26 in lung tissue. (B) Observation of lung tissue morphology and lung injury scoring statistics with hematoxylin and eosin (H&E) staining. (C) Determination of terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) positivity rate in lung tissue using TUNEL staining. (D) Measurement of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and monocyte chemoattractant protein-1 (MCP-1) levels using enzyme-linked immunosorbent assay (ELISA) assay. (E–H) Assessment of reactive oxygen species (ROS) levels (E), myeloperoxidase (MPO) activity (F), malondialdehyde (MDA) levels (G), and superoxide dismutase (SOD) activity (H) in lung tissue. (I) Estimation of endothelial integrity in vascular cross-section using cluster of differentiation 31 (CD31) fluorescence intensity. Each group consisted of 6 mice, and values are presented as mean ± standard deviation (SD), where ∗ P < 0.05 denotes significance, ∗∗ P < 0.01 denotes high significance, ∗∗∗ P < 0.001, and ns indicates non-significance. NO: normoxia; Ctrl: control; DAPI: 4′,6-diamidino-2-phenylindole; α-SMA: α-smooth muscle actin.

Article Snippet: The fluorescence intensity (FI) was measured using the Infinite F200 fluorescence ELISA reader (TECAN, Männedorf, Switzerland) at excitation and emission wavelengths of 485 and 530 nm [ ].

Techniques: Derivative Assay, Labeling, Staining, End Labeling, TUNEL Assay, Enzyme-linked Immunosorbent Assay, Activity Assay, Fluorescence, Standard Deviation, Control

Journal: Journal of Pharmaceutical Analysis

Article Title: Impact of FASN-enriched EVs on endothelial cell function in obstructive sleep apnea hypopnea syndrome

doi: 10.1016/j.jpha.2025.101251

Figure Lengend Snippet: The intervention of fatty acid synthase (FASN) improves endothelial permeability by inhibiting apoptosis of endothelial cells. (A) enzyme-linked immunosorbent assay (ELISA) assay was performed to measure the levels of endothelial function markers normoxia (NO), inorganic phosphate (Pi), and endothelin-1 (ET-1) in the peripheral blood of mice. (B, C) Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)/ cluster of differentiation 31 (CD31) double immunofluorescence staining in the dysfunctional area (B) and the statistical data (C). (D, E) Estimation of endothelial integrity in vascular cross-sections based on CD31 fluorescence intensity, with lower intensity indicating greater endothelial injury (D) and the statistical data (E). (F, G) Triple immunofluorescence staining of F4/80/α-smooth muscle actin (α-SMA)/ interleukin-6 (IL-6) to observe endothelial cells in vascular cross-sections (F) and the statistical data (G). (H, I) Lung blue dye perfusion images (H) and Evans blue leakage index (I). Tissue samples shown in each group represent typical samples, reflecting the overall trend. Each group consisted of 6 mice, and data are presented as mean ± standard deviation (SD). ∗ P < 0.05 indicates a significant difference, ∗∗ P < 0.01 indicates a highly significant difference, and ∗∗∗ P < 0.001 indicates a very significant difference. NO: normoxia; Ctrl: control; sh-NC: control shRNA; sh-FASN: FASN-targeting shRNA; CIH: chronic intermittent hypoxia; DAPI: 4′,6-diamidino-2-phenylindole.

Article Snippet: The fluorescence intensity (FI) was measured using the Infinite F200 fluorescence ELISA reader (TECAN, Männedorf, Switzerland) at excitation and emission wavelengths of 485 and 530 nm [ ].

Techniques: Permeability, Enzyme-linked Immunosorbent Assay, End Labeling, TUNEL Assay, Double Immunofluorescence Staining, Fluorescence, Immunofluorescence, Staining, Standard Deviation, Control, shRNA